Regulatory
Prm +

Part:BBa_I12006:Design

Designed by: mcnamara   Group: Antiquity   (2004-07-14)


Modified lamdba Prm promoter (repressed by 434 cI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.


Source

Bushman(1993), Shih & Gussin (1983)

References

  • Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
  • Shih, M. C. and Gussin, G. N. Mutations affectiong two different steps in transcription initiation at the phage lambda Prm promoter. Proc. Natl. Acad. Sci. USA (1983) 80, 496-500.